![]() This assay also features an internal positive control to ensure DNA of sufficient quantity and quality is used in the assay, increasing its reliability and reducing the chances of false negative results. Here, we report the development of a multiplex PCR assay targeting cytochrome oxidase I of coconut rhinoceros beetle and oriental flower beetle that can rapidly detect and distinguish between these insects. Eggs, early life stages, and fecal excrement of coconut rhinoceros beetle and oriental flower beetle are morphologically indistinguishable, thereby creating uncertainty when such specimens are discovered in the field. Confounding this program is the widespread presence of another scarab beetle on Oahu, the oriental flower beetle, Protaetia orientalis (Gory and Percheron 1833). An incipient coconut rhinoceros beetle population was recently discovered on the island of Oahu, Hawaii and is currently the target of a large, mutiagency eradication program. The coconut rhinoceros beetle, Oryctes rhinoceros (L.), is a major pest of coconut and other palm trees. The advantage of research is it can be used in integrated pest management (IPM) packages for monitoring of beetle population, and removal of beetles.Ī Multiplex PCR Assay for Differentiating Coconut Rhinoceros Beetle (Coleoptera: Scarabaeidae) From Oriental Flower Beetle (Coleoptera: Scarabaeidae) in Early Life Stages and Excrement. Aggregation pheromone is useful for attracting females. This result indicates the high population of O. rhinoceros and 9.9% other insect species (Rhynchophorus ferrugineus and Xylotrupes gideon). In 12 weeks of monitoring, the traps captured 101 insects consist of 90.1% O. The traps were observed, and the beetles trapped were counted every week. The pheromone compounds were placed in traps (buckets), hung 2 meters above the ground. Aggregation pheromones is a chemical compound containing Ethyl 4-methyl octanoate. rhinoceros that were attracted to aggregation pheromones in the field. The study aimed to monitor the population and analyze the sex ratio of O. rhinoceros attacked one of the coconuts producing villages with more than 75% of the coconut plant population O. Oryctes rhinoceros (Coleoptera: Scarabaeidae) is the most serious pest of coconut plantations in Indonesia. Once you have captured the site onto your computer, click on the directory it has been saved into and open the page called 'index' (this might also be called 'index.html').Aggregation pheromones for monitoring the coconut rhinoceros beetle (Oryctes rhinoceros) in Jerukwangi Village, Jepara, Indonesia We have found HTTRACK is quite easy to use and preserves the formatting well, though it does work best in newer versions of internet browsers. Alternatively, if you want to take a copy of the CPDT yourself, you can do this using a number of website capture tools. If you want a USB copy of the toolkit, send your address details to SPC. Getting help: as well as the resources in the CPDT, technical experts, regional agencies and NGOs can help youĪt the bottom of every page, you'll find links to credits, a glossary, and a site map that provides an overview of content in the site.Learning & teaching: resources for awareness and training.Diversity & resilience: global change, it's effects on coconut production, and ways to minimise these effects.Prevention: general approaches to prevention (biosecurity).Identification: identify possible pests or diseases using the symptoms that are observed.Those causing more severe problems are described in more detail. ![]() Coconut pests & diseases: all the possible pests and diseases affecting coconut.The toolkit is mostly targeted at helping developing Pacific Nations, who often do not have access to pest control locally and depend on outside help. The CPDT is intended to aid in basic training for the management of coconut pests and diseases in the Pacific. ![]() Welcome to the Coconut Pests and Diseases toolkit (CPDT). This toolkit has been developed as part of the Coconut Industry Development for the Pacific, funded by the European Union.
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